Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
SIN3A

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
biomaterial_provider
WiCell Research Institute
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
H1-hESC
cell organism
human
cell description
embryonic stem cells
cell karyotype
normal
cell lineage
inner cell mass
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
SIN3A_(NB600-1263)
antibody antibodydescription
Rabbit polyclonal, Immunogen: Synthetic peptide: MKRRLDDQESDVYAAQQRR, corresponding to amino acids 1-19 of Mouse mSin3A. Antibody Target: SIN3A (NB600-1263)
antibody targetdescription
Mammalian Sin 3 (mSin 3) is closely related to the yeast SIN3 repressor protein involved in the transcriptional repression of many genes. Containing 4 paired amphipathic helix domains (PAH domains), mSin 3A and mSin 3B have been shown to directly interact with several other transcriptional repressor proteins including HDAC 1, HDAC 2, RbAp 46, the methyl CpG binding protein MeCP 2, the Mad/Max heterodimer, and the corepressors silencing mediator of retinoic acid & thyroid hormone receptor (SMRT) and nuclear receptor corepressor (N-CoR).
antibody vendorname
Novus Biologicals
antibody vendorid
NB600-1263
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH001834
replicate
1

Sequenced DNA Library

library_name
GSM935289: Stanford_ChipSeq_H1-hESC_SIN3A_(NB600-1263)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
64042324
Reads aligned (%)
90.5
Duplicates removed (%)
6.1
Number of peaks
34217 (qval < 1E-05)

hg38

Number of total reads
64042324
Reads aligned (%)
92.2
Duplicates removed (%)
4.9
Number of peaks
34628 (qval < 1E-05)

Base call quality data from DBCLS SRA