Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
GM12891
Tissue
blood
Lineage
mesoderm
Description
B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed

Attributes by original data submitter

Sample

source_name
GM12891
biomaterial_provider
Coriell
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM12891
cell organism
human
cell description
B-lymphocyte, lymphoblastoid, International HapMap Project, CEPH/Utah pedigree 1463, treatment: Epstein-Barr Virus transformed
cell karyotype
normal
cell lineage
mesoderm
cell sex
M
treatment
None
treatment description
No special treatment or protocol applies
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
wgEncodeEH000709
labversion
remapped from hg18, Data paired with GM12891_IgG_Control.
replicate
1

Sequenced DNA Library

library_name
GSM935287: Stanford_ChipSeq_GM12891_Pol2_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
117700576
Reads aligned (%)
57.9
Duplicates removed (%)
8.6
Number of peaks
29998 (qval < 1E-05)

hg19

Number of total reads
117700576
Reads aligned (%)
56.9
Duplicates removed (%)
8.7
Number of peaks
31434 (qval < 1E-05)

Base call quality data from DBCLS SRA