Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RELA

Cell type

Cell type Class
Blood
Cell type
GM19099
Tissue
blood
Lineage
mesoderm
Description
lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Nigera, treatment: Epstein-Barr Virus transformed

Attributes by original data submitter

Sample

source_name
GM19099
biomaterial_provider
Coriell
lab
Stanford
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
GM19099
cell organism
human
cell description
lymphoblastoid, International HapMap Project, Yoruba in Ibadan, Nigera, treatment: Epstein-Barr Virus transformed
cell karyotype
normal
cell lineage
mesoderm
cell sex
F
treatment
TNFa
treatment description
human recombinant TNF-alpha from eBioscience [product# 14-8329-62] (Snyder)
antibody
NFKB
antibody antibodydescription
Rabbit polyclonal. Antibody Target: NFKB
antibody targetdescription
Epitope mapping at the C-terminus of NF-kappa-B p65 of human origin, recommended for detection of NFkB p65 of m, r and h origin by WB, IP, IF and ELISA
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-372
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
wgEncodeEH000778
labversion
remapped from hg18, Data paired with GM19099_IgG_Control
replicate
1

Sequenced DNA Library

library_name
GSM935273: Stanford_ChipSeq_GM19099_TNFa_NFKB_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
51533715
Reads aligned (%)
72.8
Duplicates removed (%)
4.1
Number of peaks
24441 (qval < 1E-05)

hg38

Number of total reads
51533715
Reads aligned (%)
74.3
Duplicates removed (%)
3.3
Number of peaks
23630 (qval < 1E-05)

Base call quality data from DBCLS SRA