Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Glioma

MeSH Description

Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)

Sample

source_name

Tumor (glioma)

strain

C57BL/6 transplanted into CD1 Nude

cell type

glioma propagating cells

passage

<10

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were sonicated using Covaris Sonicator and histone-DNA complexes were isolated with antibody. DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

24497358

Reads aligned (%)

96.7

Duplicates removed (%)

22.7

Number of peaks

395 (qval < 1E-05)

mm9

Number of total reads

24497358

Reads aligned (%)

96.4

Duplicates removed (%)

22.7

Number of peaks

430 (qval < 1E-05)