Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Astrocytes

MeSH Description

A class of large neuroglial (macroglial) cells in the central nervous system - the largest and most numerous neuroglial cells in the brain and spinal cord. Astrocytes (from "star" cells) are irregularly shaped with many long processes, including those with "end feet" which form the glial (limiting) membrane and directly and indirectly contribute to the BLOOD-BRAIN BARRIER. They regulate the extracellular ionic and chemical environment, and "reactive astrocytes" (along with MICROGLIA) respond to injury.

Sample

source_name

Cerebral cortex, day P5

strain

C57BL/6

cell type

astrocytes

passage

<10

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were sonicated using Covaris Sonicator and histone-DNA complexes were isolated with antibody. DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

35713643

Reads aligned (%)

95.8

Duplicates removed (%)

28.3

Number of peaks

559 (qval < 1E-05)

mm9

Number of total reads

35713643

Reads aligned (%)

95.5

Duplicates removed (%)

28.3

Number of peaks

594 (qval < 1E-05)