Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Blood
Cell type
RAJI
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type

Attributes by original data submitter

Sample

source_name
Raji cells
cell type
B cells
cancer type
Burkitt's lymphoma
chip antibody
c-Myc (Santa Cruz Biotech., sc-764)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA fragments were obtained after sonication of isolated nuclei. Histone-DNA or transcription factors-DNA complexes were isolated with appropiate antibody. For ChIPseq, 2–10 ng ChIP DNA was prepared for HiSeq2000 sequencing with TruSeq ChIP Sample Prep Kit (Illumina) following the manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
111531838
Reads aligned (%)
64.5
Duplicates removed (%)
25.8
Number of peaks
19701 (qval < 1E-05)

hg38

Number of total reads
111531838
Reads aligned (%)
66.6
Duplicates removed (%)
24.1
Number of peaks
19341 (qval < 1E-05)

Base call quality data from DBCLS SRA