GSM1976292: E2f1 DMSO ChIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
E2F1
Cell type
Cell type Class
Blood
Cell type
RAJI
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type
Attributes by original data submitter
Sample
source_name
Raji cells
cell type
B cells
cancer type
Burkitt's lymphoma
chip antibody
E2F-1 (Santa Cruz Biotech., sc-193)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA fragments were obtained after sonication of isolated nuclei. Histone-DNA or transcription factors-DNA complexes were isolated with appropiate antibody. For ChIPseq, 2–10 ng ChIP DNA was prepared for HiSeq2000 sequencing with TruSeq ChIP Sample Prep Kit (Illumina) following the manufacturer’s instructions.