Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Blood

Cell type

Lymphoblastoid cell line

Tissue

blood

Lineage

mesoderm

Description

parental cell type to lymphoblastoid cell lines

Sample

source_name

Lymphoblast Cell Lines

cell line

Epstein Barr Virus transformed Lymphoblast Cell Line

genotype/variation

expressing HA tagged EBNA3B protein

chip antibody

HA-probe Antibody F-7 (sc-7392; Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Protein A/G magnetic beads was used to capture the protein DNA complexed. The eluted protein-DNA complexes were reverse crosslinked and DNA was purified by a QIAquick PCR purification kit (28706; Qiagen). Libraries were prepared using PrepX BeadX kit from IntegenX. Briefly, DNA was end-repaired. 3' A base was added and ligated to adaptors. After PCR amplification, DNA was purified and analyzed with Illumina HiSeq.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

71646718

Reads aligned (%)

11.8

Duplicates removed (%)

67.6

Number of peaks

1751 (qval < 1E-05)

hg19

Number of total reads

71646718

Reads aligned (%)

11.7

Duplicates removed (%)

68.5

Number of peaks

1198 (qval < 1E-05)