SRX1490789
GSM1974916: D.melanogaster roX2-mutated (Odd) ChIRP-seq (with transgenic D.melanogaster roX2 with mutated stem-loops); Drosophila melanogaster; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Larvae
Cell type
3rd instar
NA
NA

Attributes by original data submitter

Sample

source_name
D.melanogaster mixed-sex wandering 3rd instar larvae
tissue
whole larvae
developmental stage
third instar larval stage
gender
mixed

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from recovered chromatin fraction by ChIRP by gentle elution with Rnase A and H, proteinase K digestion, and phenol-chloroform extraction. ChIRP-seq libraries were prepared using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina with standard TruSeq adapters.

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

dm6

Number of total reads
13861070
Reads aligned (%)
78.8
Duplicates removed (%)
70.4
Number of peaks
2161 (qval < 1E-05)

dm3

Number of total reads
13861070
Reads aligned (%)
79.3
Duplicates removed (%)
66.9
Number of peaks
3825 (qval < 1E-05)

Base call quality data from DBCLS SRA