Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Larvae
Cell type
3rd instar
NA
NA

Attributes by original data submitter

Sample

source_name
D.melanogaster mixed-sex wandering 3rd instar larvae
tissue
whole larvae
developmental stage
third instar larval stage
gender
mixed

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted from recovered chromatin fraction by ChIRP by gentle elution with Rnase A and H, proteinase K digestion, and phenol-chloroform extraction. ChIRP-seq libraries were prepared using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina with standard TruSeq adapters.

Sequencing Platform

instrument_model
NextSeq 500

dm6

Number of total reads
11968841
Reads aligned (%)
90.8
Duplicates removed (%)
53.6
Number of peaks
2097 (qval < 1E-05)

dm3

Number of total reads
11968841
Reads aligned (%)
91.0
Duplicates removed (%)
53.0
Number of peaks
2134 (qval < 1E-05)

Base call quality data from DBCLS SRA