Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Larvae

Cell type

3rd instar

NA

NA

Sample

source_name

D.melanogaster mixed-sex wandering 3rd instar larvae

tissue

whole larvae

developmental stage

third instar larval stage

gender

mixed

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted from recovered chromatin fraction by ChIRP by gentle elution with Rnase A and H, proteinase K digestion, and phenol-chloroform extraction. ChIRP-seq libraries were prepared using NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina with standard TruSeq adapters.

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

14143419

Reads aligned (%)

89.5

Duplicates removed (%)

62.8

Number of peaks

1905 (qval < 1E-05)

dm3

Number of total reads

14143419

Reads aligned (%)

90.5

Duplicates removed (%)

61.8

Number of peaks

2436 (qval < 1E-05)