Antigen

Antigen Class

TFs and others

Antigen

Ep300

Cell type

Cell type Class

Blood

Cell type

HPC-7

NA

NA

Sample

source_name

HPC7

cell line

hematopoietic cell line HPC7

protocol

shSCR

chip antibody

p300 (santa cruz sc-585 X, lot j2214)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were harvested, washed with PBS and crosslinked with 1% Formaldehyde for 30 min at 37C. Libraries were constructed with KAPA Hyper Prep Kit and Multiplexed with NextFlex Barcodes from Biooscientific

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

51862021

Reads aligned (%)

87.9

Duplicates removed (%)

57.8

Number of peaks

2588 (qval < 1E-05)

mm9

Number of total reads

51862021

Reads aligned (%)

87.6

Duplicates removed (%)

57.9

Number of peaks

2583 (qval < 1E-05)