Antigen

Antigen Class

TFs and others

Antigen

Med12

Cell type

Cell type Class

Blood

Cell type

HPC-7

NA

NA

Sample

source_name

HPC7

cell line

hematopoietic cell line HPC7

chip antibody

Med12 (bethyl-A300-774A, lot 1)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were harvested, washed with PBS and crosslinked with 1% Formaldehyde for 30 min at 37C. Libraries were constructed with KAPA Hyper Prep Kit and Multiplexed with NextFlex Barcodes from Biooscientific

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

37011981

Reads aligned (%)

93.8

Duplicates removed (%)

48.6

Number of peaks

2125 (qval < 1E-05)

mm9

Number of total reads

37011981

Reads aligned (%)

93.4

Duplicates removed (%)

48.7

Number of peaks

2137 (qval < 1E-05)