Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

HSPCs

cell type

hematopoietic stem and progenitor cells

genotype/variation

KO

strain

129Sv/B6

chip antibody 1

none (input)

chip antibody 2

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells from bone marrow were isolated from mice and eriched for c-Kit+ cells,crosslinked for 10 minutes with 1% formaldehyde and stained with Lineage antibodies (CD11b, Gr1, Ter119, B220, CD4, CD8), Sca1 and c-Kit and then sorted and snap frozen Libraries were generated as previously published (Lara Astiaso et al, 2014) Briefly, sonicated chromatin was immunoprecipitated with H3 and libraries were generated on chromatin bound to beads. Libaries with multiplexed with NextFlex Barcoded primers from Bioo Scientific. ChIp with specific histone modifications were performed with barcoded chromatin and released DNA was amplified with ilumina standard primers

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

13316599

Reads aligned (%)

98.1

Duplicates removed (%)

14.0

Number of peaks

369 (qval < 1E-05)

mm9

Number of total reads

13316599

Reads aligned (%)

97.9

Duplicates removed (%)

14.2

Number of peaks

379 (qval < 1E-05)