Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tfe3

Cell type

Cell type Class
Blood
Cell type
RAW 264.7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
RAW_Starvation_ChIPSeq_activated_macrophage
cell line
RAW 264.7
cell type
Activated Macrophage
chip antibody
TFE3: Sigma, Catalog: HPA023881-100ul, Lot: E96562

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, samples were sonicated and lysates were cleared from sonicated nuclei and used for immunoprecipitation. Cells were cross-linked in 1% formaldehyde and processed according to Myers Lab ChIP-seq protocol for adherent cells. Purified DNA was processed according to manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
40695760
Reads aligned (%)
96.8
Duplicates removed (%)
15.5
Number of peaks
6695 (qval < 1E-05)

mm9

Number of total reads
40695760
Reads aligned (%)
96.6
Duplicates removed (%)
15.5
Number of peaks
6684 (qval < 1E-05)

Base call quality data from DBCLS SRA