Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NFE2L2

Cell type

Cell type Class
Lung
Cell type
BEAS-2B
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
BEAS-2B
treatment
SFN
cell line
BEAS-2B
chip antibody
rabbit monoclonal anti-NRF2 (Epitomics, Clone ID EP1808Y)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and NRF2-DNA complexes were isolated with antibody (Epitomics, Clone ID EP1808Y). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit. Briefly, DNA was end-repaired using a combination of T4 DNA polymerase, E. coli DNA Pol I large fragment (Klenow polymerase) and T4 polynucleotide kinase. The blunt, phosphorylated ends were treated with Klenow fragment (32 to 52 exo minus) and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters which have a single 'T' base overhang at the 3’ end. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
13241133
Reads aligned (%)
19.0
Duplicates removed (%)
78.6
Number of peaks
287 (qval < 1E-05)

hg38

Number of total reads
13241133
Reads aligned (%)
20.8
Duplicates removed (%)
76.8
Number of peaks
243 (qval < 1E-05)

Base call quality data from DBCLS SRA