Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

T-47D

Primary Tissue

Breast

Tissue Diagnosis

Adenocarcinoma Ductal

Sample

source_name

Human breast cancer cell line

cell line

T47D

condition

sgRNA_enh588

procedure

ChIP-seq

antibody

anti-ERa (SC-543; Santa Cruz)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin Immunoprecipitation was performed as described (Zwart W et al. EMBO J. 2011, 30:4764-76) : 10ug of antibody was used, with 100ml of Protein A magnetic beads. Antibody used was raised to detect Estrogen Receptor alpha (HC-20, SC-543; Santa Cruz). DNA was amplified as described (Ross-Innes CS et al. Nature 2012;481(7381):389-93). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

28009591

Reads aligned (%)

89.6

Duplicates removed (%)

2.8

Number of peaks

6341 (qval < 1E-05)

hg19

Number of total reads

28009591

Reads aligned (%)

88.9

Duplicates removed (%)

4.3

Number of peaks

6268 (qval < 1E-05)