Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
MEF
strain
CRL-2977
cell type
MEF
stress
background control
chip antibody
Rabbit IgG (Santa Cruz, Catalog# sc-806, Lot# F0908)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, samples were sonicated and lysates were cleared from sonicated nuclei and used for immunoprecipitation. For ChIPSeq, cells were cross-linked in 1% formaldehyde and processed according to Myers Lab ChIP-seq protocol for adherent cells. Purified DNA was processed according to manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
40404815
Reads aligned (%)
83.7
Duplicates removed (%)
22.5
Number of peaks
570 (qval < 1E-05)

mm9

Number of total reads
40404815
Reads aligned (%)
83.5
Duplicates removed (%)
22.5
Number of peaks
610 (qval < 1E-05)

Base call quality data from DBCLS SRA