Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Epidermis
Cell type
UACC-257
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
human melanoma cell lines
cell line
UACC257
cell type
melanoma
passages
long term culture
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the methods of T. I. Lee, S. E. Johnstone, R. A. Young, Chromatin immunoprecipitation and microarray-based analysis of protein location. Nat Protoc. 1, 729–748 (2006). Libraries were prepared using the NEBNext Multiplex Oligos for Illumina kit (NEB) and run on an Illumina Hi-Seq2000.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
27737873
Reads aligned (%)
99.4
Duplicates removed (%)
1.7
Number of peaks
940 (qval < 1E-05)

hg19

Number of total reads
27737873
Reads aligned (%)
98.7
Duplicates removed (%)
3.1
Number of peaks
965 (qval < 1E-05)

Base call quality data from DBCLS SRA