Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Pax5

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
PSU-m
lab description
Hardison - Penn State University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
PAX5_(N-15)
antibody antibodydescription
rabbit polyclonal antibody; epitope is a synthetic peptide: MDLEKNYPTPRTSRT, corresponding to amino acids 1-15 of Human PAX5, cross-reacts with human and mouse PAX5. Antibody Target: PAX5
antibody targetdescription
The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B cell differentiation.
antibody vendorname
Abcam
antibody vendorid
ab12000
age
immortalized
age description
Immortal cells
control
Input
control description
Control signal which may be subtracted from experimental raw signal before peaks are called.
controlid
wgEncodeEM001923
replicate
1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM923584: PSU_ChipSeq_CH12_PAX5_(N-15)
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
106614163
Reads aligned (%)
93.6
Duplicates removed (%)
11.2
Number of peaks
996 (qval < 1E-05)

mm9

Number of total reads
106614163
Reads aligned (%)
93.5
Duplicates removed (%)
11.1
Number of peaks
1153 (qval < 1E-05)

Base call quality data from DBCLS SRA