GSM1949005: ChIP-seq SMAD3 H1 hESC; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMAD3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA
Attributes by original data submitter
Sample
source_name
H1 human Embryonic stem cells (hESC)
cell line
H1
cell type
H1 hESC
state
cultured in mTESR1
antibody
SMAD3, Abcam, AB28379
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP, cells were crosslinked with 1% formaldehyde for 10 minutes prior to sonication to solubulize the chromatin. The sheared chromatin extracts containing DNA fragments with an average size of 500 bp were immunoprecipitated using antibody against SMAD3. Purified immunoprecipitated DNA samples were prepared for sequencing using the Truseq ChIP kit from Illunima. ChIP-seq libraries were prepared according to instructions accompanying the TruSeq ChIP Kit and barcoded for sequencing.