ChIP-enriched DNA was obtaind by using ChIP-IT Express Enzymatic (Active Motif, Cat No. 53009) and anti-H1T rabbit polyclonal antibody The libraries for massive parallel sequencing were prepared with the TruSeq DNA Sample Preparation v2 Kit (Illumina) according to the manufacturer's instructions. Briefly, 50 ng of immunoprecipiated DNA or input DNA were end repaired, 3’ adenylated and ligated with adapter oligos. DNA fragments within the range of 120~500 bp were purified using AMpure XP beads (Beckman Coulter) and amplified by PCR. Library DNA was electrophoresed in 2% agarose gel and subsequently purified with a Zymoclean Gel DNA Recovery Kit (Zymo Reserch). Libraries were quantified by BioAnalyzer2000 (Agilent). Sequencing was performed with Illumina HiSeq 2000 systems