GSM1948914: Input MDAMB231; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Breast
Cell type
MDA-MB-231
Primary Tissue
Breast
Site of Extraction
Effusion, Pleural
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Input_MDAMB231
cell line
MDA-MB-231
cell type
Breast adenocarcinoma
chip-antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-enriched DNA was obtaind by using ChIP-IT Express Enzymatic (Active Motif, Cat No. 53009) and anti-H1T rabbit polyclonal antibody The libraries for massive parallel sequencing were prepared with the TruSeq DNA Sample Preparation v2 Kit (Illumina) according to the manufacturer's instructions. Briefly, 50 ng of immunoprecipiated DNA or input DNA were end repaired, 3’ adenylated and ligated with adapter oligos. DNA fragments within the range of 120~500 bp were purified using AMpure XP beads (Beckman Coulter) and amplified by PCR. Library DNA was electrophoresed in 2% agarose gel and subsequently purified with a Zymoclean Gel DNA Recovery Kit (Zymo Reserch). Libraries were quantified by BioAnalyzer2000 (Agilent). Sequencing was performed with Illumina HiSeq 2000 systems