Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Nfe2l2

Cell type

Cell type Class
Blood
Cell type
Macrophages
MeSH Description
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Attributes by original data submitter

Sample

source_name
peritoneal macrophage
cell type
peritoneal macrophage
genotype
Wild type
chip antibody
Nrf2 (Cell Signaling Technology; D1Z9C)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were fixed with 1% formaldehyde for 10 min at room temperature and subsequently quenched with 0.125-M glycine. ChIP was performed with anti-Nrf2 antibody (Cell Signaling Technology; D1Z9C).The antibody incubation were pereformed overnight at 4℃. The DNA libraries were prepared from 1.5 or 2 ng of ChIP and input samples quantified with Qubit Fluorometer (Life Technologies), using Mondrian SP+ and Ovation SP Ultralow DR Multiplex System (TaKaRa).braries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
31758066
Reads aligned (%)
98.0
Duplicates removed (%)
30.9
Number of peaks
981 (qval < 1E-05)

mm9

Number of total reads
31758066
Reads aligned (%)
97.8
Duplicates removed (%)
30.9
Number of peaks
972 (qval < 1E-05)

Base call quality data from DBCLS SRA