Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me1

Cell type

Cell type Class
Neural
Cell type
Neural Stem Cells
MeSH Description
Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Attributes by original data submitter

Sample

source_name
neural stem cell
strain
C57BL/6
development stage
--
chip antibody
H3K4me1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linking, chromatin isolation, sonication and immunoprecipitation were performed using MAGnifyTM Chromatin Immunoprecipitation System (Invitrogen) following the manufacturer’s instructions. Library was constructed using KAPA HyperPlus Library Preparation Kit (Cat. No. kk8510)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
12654760
Reads aligned (%)
99.5
Duplicates removed (%)
5.3
Number of peaks
3278 (qval < 1E-05)

mm9

Number of total reads
12654760
Reads aligned (%)
99.4
Duplicates removed (%)
5.3
Number of peaks
3259 (qval < 1E-05)

Base call quality data from DBCLS SRA