Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Neural

Cell type

Neural Stem Cells

MeSH Description

Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.

Sample

source_name

neural stem cell

strain

C57BL/6

development stage

--

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linking, chromatin isolation, sonication and immunoprecipitation were performed using MAGnifyTM Chromatin Immunoprecipitation System (Invitrogen) following the manufacturer’s instructions. Library was constructed using KAPA HyperPlus Library Preparation Kit (Cat. No. kk8510)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

15869894

Reads aligned (%)

99.5

Duplicates removed (%)

2.4

Number of peaks

128 (qval < 1E-05)

mm9

Number of total reads

15869894

Reads aligned (%)

99.5

Duplicates removed (%)

2.3

Number of peaks

99 (qval < 1E-05)