Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Erythroblasts

MeSH Description

Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.

Sample

source_name

G-CSF morbilized CD34+-derived erythrblasts

differentiation stage

Proerythroblasts~Early basophilic erythroblasts

culture condition

EDM-II (Day8)

mouse strain

NA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Five million cells were fixed with 1% formaldehyde at RT for 20 min and chromatin was collected using a truChIP High Cell Chromatin Shearing Kit with SDS (Covaris). ChIP-Seq libraries were generated using a Ovation Ultralow DR Multiplex System 1-8 (NuGEN)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

59084007

Reads aligned (%)

86.8

Duplicates removed (%)

83.0

Number of peaks

1234 (qval < 1E-05)

hg19

Number of total reads

59084007

Reads aligned (%)

86.1

Duplicates removed (%)

84.8

Number of peaks

1152 (qval < 1E-05)