Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
HUDEP-2
NA
NA

Attributes by original data submitter

Sample

source_name
WT HUDEP-2
differentiation stage
Early basophilic erythroblasts
culture condition
SFEM doxycycline+ (Day0)
mouse strain
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Five million cells were fixed with 1% formaldehyde at RT for 20 min and chromatin was collected using a truChIP High Cell Chromatin Shearing Kit with SDS (Covaris). ChIP-Seq libraries were generated using a Ovation Ultralow DR Multiplex System 1-8 (NuGEN)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
62847822
Reads aligned (%)
93.5
Duplicates removed (%)
58.8
Number of peaks
9552 (qval < 1E-05)

hg19

Number of total reads
62847822
Reads aligned (%)
92.7
Duplicates removed (%)
59.7
Number of peaks
9022 (qval < 1E-05)

Base call quality data from DBCLS SRA