Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREB1

Cell type

Cell type Class
Blood
Cell type
KG-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
KG1 cells
cell line
acute myeloid leukemia cell line KG1
treatment
DMSO (0.1%, 6h)
chip ab
CREB (Millipore, 17-600)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin were digested by Micrococcal nuclease and then sonicated using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) following themanufacturer’s protocol. CREB-DNA complexes were isolated with antibody. Libraries were prepared using the Illumina Truseq RNA samples prep kit per manufacturer’s instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
41625469
Reads aligned (%)
87.9
Duplicates removed (%)
7.3
Number of peaks
2231 (qval < 1E-05)

hg38

Number of total reads
41625469
Reads aligned (%)
89.2
Duplicates removed (%)
6.4
Number of peaks
2290 (qval < 1E-05)

Base call quality data from DBCLS SRA