GSM1937941: DMSO CREB ChIPSeq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CREB1
Cell type
Cell type Class
Blood
Cell type
KG-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
KG1 cells
cell line
acute myeloid leukemia cell line KG1
treatment
DMSO (0.1%, 6h)
chip ab
CREB (Millipore, 17-600)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin were digested by Micrococcal nuclease and then sonicated using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) following themanufacturer’s protocol. CREB-DNA complexes were isolated with antibody. Libraries were prepared using the Illumina Truseq RNA samples prep kit per manufacturer’s instructions.