Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
0-4h embryos
NA
NA

Attributes by original data submitter

Sample

source_name
embryos
genotype
SuUR mutant
chip antibody
Abcam anti-H3K27me3 (6002)
tissue
0-4h embryos

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
25 larval salivary glands, 25 larval brains or about 20 ul of 0-4h embryos were used as starting material. Abcam anti-H3K27me3 antibodies (6002) were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using Illumina Nextera protocol.

Sequencing Platform

instrument_model
Illumina MiSeq

dm3

Number of total reads
791594
Reads aligned (%)
30.8
Duplicates removed (%)
1.4
Number of peaks
30 (qval < 1E-05)

dm6

Number of total reads
791594
Reads aligned (%)
30.6
Duplicates removed (%)
1.7
Number of peaks
16 (qval < 1E-05)

Base call quality data from DBCLS SRA