Curated Sample Data


Genome
dm3
Antigen Class
Histone
Antigen
H3K27me3
Cell type Class
Embryo
Cell type
0-4h embryos

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
embryos
genotype
SuUR mutant
chip antibody
Abcam anti-H3K27me3 (6002)
tissue
0-4h embryos

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
25 larval salivary glands, 25 larval brains or about 20 ul of 0-4h embryos were used as starting material. Abcam anti-H3K27me3 antibodies (6002) were used for chromatin immunoprecipitation. Chromatin was sheared using BioRuptor instrument (Diagenode). DNA from precipitated material and input were used for library preparation using Illumina Nextera protocol.

Platform Information


instrument_model
Illumina MiSeq

External Database Query

Logs in read processing pipeline


Number of total reads
791594
Reads aligned (%)
30.8
Duplicates removed (%)
1.4
Number of peaks
30 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA