ChIP-Atlas: SRX1426084

Antigen

Cell type Class: Epidermis
Cell type: Epidermis
MeSH Description: The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Nano-ChIPseqs were conducted as previously reported (Adli and Bernstein, Nature Protocol, 2011) and libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303). Briefly, ChIP DNA was primed and amplified using designed primers for 15 cycles and digested with BciV1 restriction enzyme. After digestion, the DNA was ligated with Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 17 cycles and library fragments of 200-500 bp (insert plus adaptor and PCR primer sequences) were isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Genome Analyzer following the manufacturer's protocols.