Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tcf12

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
C2C12
biomaterial_provider
Barbara Wold lab
lab
Caltech-m
lab description
Wold - Califonia Institute of Technology
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
C2C12
cell organism
mouse
cell description
Myoblast cell line derived from thigh muscle of C3H mice after crush injury
cell sex
F
antibody
TCF12
antibody description
HEB (A-20) Rabbit polyclonal IgG, 200 ?g/ml. Epitope mapping within the C-terminus of HEB of human origin. Antibody Target: TCF12
antibody targetdescription
The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-357
control
Control_50bp
control description
This data represents a control being compared to experiments using read length of 50 bp
treatment
EqS_2.0pct_60hr
treatment description
60 h with 2.0% Equine Serum and Insulin (Wold)
age
immortalized
age description
Immortal cells
control
Control_50bp
control description
This data represents a control being compared to experiments using read length of 50 bp
controlid
11610
labexpid
11719
labversion
Myocyte
labversion description
Myocyte
replicate
1
strain
C3H
strain description
Strain C3H, Very related to C57BL/6

Sequenced DNA Library

library_name
GSM915178: Caltech_ChipSeq_C2C12_TCF12_Control_50bp_EqS_2.0pct_60hr
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
25490531
Reads aligned (%)
86.8
Duplicates removed (%)
56.2
Number of peaks
14619 (qval < 1E-05)

mm9

Number of total reads
25490531
Reads aligned (%)
86.7
Duplicates removed (%)
56.4
Number of peaks
14609 (qval < 1E-05)

Base call quality data from DBCLS SRA