Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Srf

Cell type

Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS

Attributes by original data submitter

Sample

source_name
C2C12
biomaterial_provider
Barbara Wold lab
lab
Caltech-m
lab description
Wold - Califonia Institute of Technology
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
C2C12
cell organism
mouse
cell description
Myoblast cell line derived from thigh muscle of C3H mice after crush injury
cell sex
F
antibody
SRF
antibody description
Rabbit polyclonal. Antibody Target: SRF
antibody targetdescription
Serum response transcription factor
antibody vendorname
Santa Cruz Biotech
antibody vendorid
sc-335
control
Control_32bp
control description
This data represents a control being compared to experiments using read length of 32 bp
treatment
EqS_2.0pct_24hr
treatment description
24 h with 2.0% Equine Serum and Insulin (Wold)
age
immortalized
age description
Immortal cells
control
Control_32bp
control description
This data represents a control being compared to experiments using read length of 32 bp
controlid
10007
labexpid
10335
labversion
Myocyte
labversion description
Myocyte
replicate
1
strain
C3H
strain description
Strain C3H, Very related to C57BL/6

Sequenced DNA Library

library_name
GSM915168: Caltech_ChipSeq_C2C12_SRF_Control_32bp_EqS_2.0pct_24hr
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
27760901
Reads aligned (%)
79.5
Duplicates removed (%)
4.3
Number of peaks
6107 (qval < 1E-05)

mm9

Number of total reads
27760901
Reads aligned (%)
79.4
Duplicates removed (%)
4.5
Number of peaks
6113 (qval < 1E-05)

Base call quality data from DBCLS SRA