Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
KMT2A

Cell type

Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Leukaemia cell line (SEM)
cell line
SEM
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
MLL (Bethyl, A300-086A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
29362705
Reads aligned (%)
176.6
Duplicates removed (%)
9.7
Number of peaks
12731 (qval < 1E-05)

hg38

Number of total reads
29362705
Reads aligned (%)
178.3
Duplicates removed (%)
9.5
Number of peaks
12884 (qval < 1E-05)

Base call quality data from DBCLS SRA