Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
SEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
Leukaemia cell line (SEM)
cell line
SEM
cell type
Paediatric pro B-cell line derived from ALL with t(4;11)(q21;q23) translocation
chip antibody
H3K27ac (Diagenode, pAb-196-050, lot A1847-001P)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed cells were lysed in 1% SDS lysis buffer. Samples were sonicated and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's protocol accompanying the DNA Library Prep Master Mix Set (Part# E6040).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
31769527
Reads aligned (%)
97.0
Duplicates removed (%)
5.1
Number of peaks
20061 (qval < 1E-05)

hg19

Number of total reads
31769527
Reads aligned (%)
96.2
Duplicates removed (%)
5.3
Number of peaks
19750 (qval < 1E-05)

Base call quality data from DBCLS SRA