GSM1933886: pNanog1-Dox+NgIP; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NANOG
Cell type
Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
cultured LNCaP prostate cancer cells
cell line
LNCaP
cell type
prostate cancer cells
genotype/variation
NANOG1 overexpression
cultured with
without doxycycline for 4 days
chip antibody
NANOG IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After formaldehyde fixation, lysed cell DNA was sheared by sonication and immunoprecipitated with an anti-NANOG antibody (Santa Cruz, H-155) vs IgG control and protein-A beads overnight. ChIP libraries were prepared for sequencing using standard Illumina protocols