Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Smc3

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
SMC3_(ab9263)
antibody antibodydescription
Rabbit polyclonal raised against synthetic peptide representing a portion within the last 100 amino acids of the human Structural Maintenance of Chromosomes-3 conjugated to KLH. Antibody Target: SMC3
antibody targetdescription
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication.
antibody vendorname
Abcam
antibody vendorid
ab9263
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
age
immortalized
age description
Immortal cells
control
IgG-rab
control description
Input signal from Normal Rabbit IgG ChIP-seq.
controlid
CH12/None/Input/IgG-rab
replicate
1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM912930: Stanford_ChipSeq_CH12_SMC3_(ab9263)_IgG-rab
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
53505231
Reads aligned (%)
94.2
Duplicates removed (%)
15.4
Number of peaks
53538 (qval < 1E-05)

mm9

Number of total reads
53505231
Reads aligned (%)
94.0
Duplicates removed (%)
15.4
Number of peaks
53545 (qval < 1E-05)

Base call quality data from DBCLS SRA