Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Tbp

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
TBP
antibody antibodydescription
Mouse monoclonal. Immunogen is synthetic peptide conjugated to KLH derived from within residues 1 - 100 of HumanTATA binding protein TBP. Antibody Target: TBP
antibody targetdescription
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription.
antibody vendorname
abcam
antibody vendorid
ab62126
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
age
immortalized
age description
Immortal cells
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
CH12/None/Input/IgG-mus
replicate
1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM912900: Stanford_ChipSeq_CH12_TBP_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
51901920
Reads aligned (%)
65.0
Duplicates removed (%)
18.1
Number of peaks
21727 (qval < 1E-05)

mm9

Number of total reads
51901920
Reads aligned (%)
64.8
Duplicates removed (%)
18.1
Number of peaks
21693 (qval < 1E-05)

Base call quality data from DBCLS SRA