Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Blood
Cell type
CH12
Tissue
Blood
Lineage
cellLine
Description
B-cell lymphoma (GM12878 analog)

Attributes by original data submitter

Sample

source_name
CH12
biomaterial_provider
Weissman lab
lab
Stanford-m
lab description
Snyder - Stanford University
datatype
ChipSeq
datatype description
Chromatin IP Sequencing
cell
CH12
cell organism
mouse
cell description
B-cell lymphoma (GM12878 analog)
cell sex
F
antibody
Pol2
antibody antibodydescription
mouse monoclonal, clone 8WG16. Antibody Target: POL2
antibody targetdescription
RNA Polymerase II
antibody vendorname
Covance
antibody vendorid
MMS-126R
treatment
None
treatment description
No special treatment or protocol applies
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
age
immortalized
age description
Immortal cells
control
IgG-mus
control description
Input signal from Normal Mouse IgG ChIP-seq.
controlid
CH12/None/Input/IgG-mus
replicate
1
strain
B10.H-2aH-4bp/Wts
strain description
Derived by inbreeding from selected F2 progeny of B10.A X B10.129

Sequenced DNA Library

library_name
GSM912891: Stanford_ChipSeq_CH12_Pol2_IgG-mus
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

mm10

Number of total reads
40468050
Reads aligned (%)
83.2
Duplicates removed (%)
19.0
Number of peaks
28104 (qval < 1E-05)

mm9

Number of total reads
40468050
Reads aligned (%)
83.0
Duplicates removed (%)
19.1
Number of peaks
28077 (qval < 1E-05)

Base call quality data from DBCLS SRA