Antigen

Antigen Class

TFs and others

Antigen

Med1

Cell type

Cell type Class

Blood

Cell type

MLL-AF9 leukemic cell

NA

NA

Sample

source_name

MLL-AF9/NrasG12D murine cell line

strain

C57BL/6

cell type

Acute myeloid leukemia

genotype

MLL-AF9/NrasG12D

treatment

DMSO for 2 hours

antibody

Med1 (A300-793A; Bethyl)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with appopriate sizes were prepared for sequencing. These libraries were made following the instructions of illumina Truseq DNA sample prep kit v2. These libraries were made following the instructions of illumina Truseq DNA sample prep kit v2.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

109811410

Reads aligned (%)

95.2

Duplicates removed (%)

50.9

Number of peaks

41764 (qval < 1E-05)

mm9

Number of total reads

109811410

Reads aligned (%)

95.0

Duplicates removed (%)

50.9

Number of peaks

41694 (qval < 1E-05)