Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ARNTL

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
U2OS cells stably expressing Bmal1:Luc
cell line
osteosarcoma cell line U2OS stably expressing Bmal1:Luc
agent
DMSO
chip antibody
anti-BMAL1 (ab3350; Abcam)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-Seq: Standard RNA extraction with Zymo Direct-zol™ RNA kits Illumina TrueSeq platform compatible dual-indexed multiplex library protocol was followed.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
16115693
Reads aligned (%)
86.2
Duplicates removed (%)
17.6
Number of peaks
413 (qval < 1E-05)

hg38

Number of total reads
16115693
Reads aligned (%)
86.9
Duplicates removed (%)
17.3
Number of peaks
565 (qval < 1E-05)

Base call quality data from DBCLS SRA