Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
experiment
Low_input_K562/Exp004-2
histone modification
H3K4me3
cell number
100000c
mnase units
150u
treatment
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
2211706
Reads aligned (%)
78.3
Duplicates removed (%)
4.5
Number of peaks
7543 (qval < 1E-05)

hg19

Number of total reads
2211706
Reads aligned (%)
78.2
Duplicates removed (%)
4.5
Number of peaks
7522 (qval < 1E-05)

Base call quality data from DBCLS SRA