Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
experiment
KDM5-C70/Exp015-5
histone modification
H3K4me3
mnase units
150u
treatment
KDM5C70

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
3331481
Reads aligned (%)
94.3
Duplicates removed (%)
4.9
Number of peaks
9448 (qval < 1E-05)

hg19

Number of total reads
3331481
Reads aligned (%)
94.2
Duplicates removed (%)
5.0
Number of peaks
9410 (qval < 1E-05)

Base call quality data from DBCLS SRA