Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous

Attributes by original data submitter

Sample

source_name
K562
experiment
KDM5-C70/Exp015-5
histone modification
H3
mnase units
30u
treatment
DMSO

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
4011450
Reads aligned (%)
82.7
Duplicates removed (%)
6.3
Number of peaks
878 (qval < 1E-05)

hg19

Number of total reads
4011450
Reads aligned (%)
80.6
Duplicates removed (%)
7.8
Number of peaks
856 (qval < 1E-05)

Base call quality data from DBCLS SRA