Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

K-562

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Chronic Myelogenous

Sample

source_name

K562

experiment

KDM5-C70/Exp015-5

histone modification

H3

mnase units

30u

treatment

DMSO

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell lysis immediately followed by MNase digestion of native chromatin, T7-adapter ligation, pool-and-split multiplexing, immunoprecipitation and magnetic bead isolation of immunoprecipitated DNA. Mint-ChIP: In vitro transcription, reverse transcription, PCR (see manuscript).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

1472390

Reads aligned (%)

94.8

Duplicates removed (%)

7.1

Number of peaks

175 (qval < 1E-05)

hg19

Number of total reads

1472390

Reads aligned (%)

92.3

Duplicates removed (%)

8.6

Number of peaks

176 (qval < 1E-05)