Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Pluripotent stem cell

Cell type

mESC derived neural cells

NA

NA

Sample

source_name

neural progenitor cells

strain

R1

cell type

ES-derived neural progenitor cells

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepped using 5ng of ChIP material and the Kapa Hyper Prep Kit for Illumina Sequencing (Kapa Biosystems) according to manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

47647908

Reads aligned (%)

93.7

Duplicates removed (%)

41.8

Number of peaks

491 (qval < 1E-05)

mm9

Number of total reads

47647908

Reads aligned (%)

93.5

Duplicates removed (%)

41.8

Number of peaks

554 (qval < 1E-05)