Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic stem cells
strain
R1
cell type
ES
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepped using 5ng of ChIP material and the Kapa Hyper Prep Kit for Illumina Sequencing (Kapa Biosystems) according to manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
41790621
Reads aligned (%)
94.2
Duplicates removed (%)
18.5
Number of peaks
407 (qval < 1E-05)

mm9

Number of total reads
41790621
Reads aligned (%)
94.1
Duplicates removed (%)
18.5
Number of peaks
386 (qval < 1E-05)

Base call quality data from DBCLS SRA