Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

Dendritic cells

strain

C57BL/6

cell type

Bone marrow derived dendritic cells

agent

diluent

Sex

female

age

10 weeks

chip antibody

Rabbit IgG (Cat. 2729, Cell Signaling)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin (~200 ng of DNA) was sonicated into 200-500 bp fragments, DNA ends were repaired with polynucleotide kinase and Klenow enzyme and treated with Taq polymerase for generation of a protruding 3′ “A” nucleotide for adaptor ligation. Libraries were prepared with the Illumina ChIP-seq Sample Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

25882793

Reads aligned (%)

98.7

Duplicates removed (%)

8.9

Number of peaks

530 (qval < 1E-05)

mm9

Number of total reads

25882793

Reads aligned (%)

98.6

Duplicates removed (%)

9.0

Number of peaks

577 (qval < 1E-05)