Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Su(var)205

Cell type

Cell type Class
Cell line
Cell type
OSC
Tissue Source
ovary
Developmental Stage
adult stage

Attributes by original data submitter

Sample

source_name
Ovarian Somatic Cells (OSC)
chip-seq antibody
anti-HP1, Covance (#291C)
genotype/variation
291C Ab in GFP-KD

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
for RNA-seq and small RNA-seq the total RNA was extracted using TRIZOL Reagent (Invitrogen) for ChIP chromatin was prepared and extracted as in Lee et all 2006 small-RNAs were cloned as described in Senti et al 2015

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
27782670
Reads aligned (%)
97.3
Duplicates removed (%)
28.0
Number of peaks
10440 (qval < 1E-05)

dm6

Number of total reads
27782670
Reads aligned (%)
96.6
Duplicates removed (%)
36.1
Number of peaks
0 (qval < 1E-05)

Base call quality data from DBCLS SRA