GSM1915119: Input Proliferating IMR90; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
IMR90 human fibrobasts
cell line
IMR90
cell type
fibrobasts
antibody
none (input)
treatment
proliferating
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Five to 30 million IMR90 cells were crosslinked for 10 minutes using 1 % formaldehyde, followed by quenching with 0.125M glycine for 10 minutes. To prepare chromatin from crosslinked-senescent cells, aliquots of less than 1 million cells were kept in individual eppendorf tubes for sonication to maximize the chromatin fragmentation efficiency. After purifying immunoprecipitated DNA, a TruSeq ChIP Sample Prep Kit (Illumina) was used to construct the ChIP-Seq library following the manufacturer's protocol except for amplifying the adaptor-ligated library for 15 cycles. ChIP-Seq libraries were sequenced using an illumina HiSeq 2000 platform with single end reads of 50 bases. Four libraries at equal molarity were pooled to aim for a sequencing depth of 20-40 million aligned reads per sample.